产品简介

品牌 货号 规格 供货周期 应用领域

其他品牌	A00186
100ug	一周
生物产业

His抗体A00186 Tests perbed at GenScript show that the antibody can also recognize 4xHis- and 5xHis-tags.

详情介绍

一、His抗体A00186产品介绍：

Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. However, since 6XHis-tag is poorly immunogenic, it needs to be conjugated to KLH or some other carrier as an immunogen. After hundreds of selection cycles, researchers at GenScript successfully isolated an antibody against His-tag.

THE™ His Tag Antibody, mAb, Mouse (subtype IgG1) has very high affinity. Tests perbed at GenScript show that the antibody can also recognize 4xHis- and 5xHis-tags. This means that even if the 6xHis-tag is only partially exposed, it will still be recognized and bound by this antibody. 

THETM His Tag mAb is produced from mice ascites and purified by protein A affinity column. This antibody recognizes native as well as denatured bs of synthetic polyhistidine and polyhistidine-tagged fusion proteins. The product reacts with fusion proteins expressed in bacteria, insect cells, and mammalian cells. THETM His Tag mAb recognizes His tags placed at N-terminal, C-terminal, and internal regions of fusion proteins. 

THETM His Tag mAb can be used in Western blot analyses, Dot blot analyses, ELISA, immunofluorescent staining, and flow cytometry of cultured cells.
 

中文：

特异于6个组氨酸标签的单克隆抗体可以极大地提高几种不同免疫分析的有效性，帮助研究人员识别、检测和纯化细菌、昆虫细胞和哺乳动物细胞中的多组氨酸融合蛋白。然而，由于6XHis标签的免疫原性较差，因此需要与KLH或其他载体结合作为免疫原。经过数百个选择周期后，GenScript的研究人员成功地分离出一种针对他的标签的抗体。

这个™ His标记抗体mAb与小鼠（IgG1亚型）具有很高的亲和力。在GenScript上进行的测试显示抗体也能识别4xHis和5xHis标签。这意味着即使6xHis标签仅部分暴露，它仍将被该抗体识别和结合。

THTM His标记单克隆抗体由小鼠腹水制备，并通过蛋白A亲和柱纯化。该抗体识别天然和变性形式的合成多组氨酸和多组氨酸标记的融合蛋白。该产物与细菌、昆虫细胞和哺乳动物细胞中表达的融合蛋白反应。THTM-His标签单克隆抗体识别位于融合蛋白N端、C端和内部区域的His标签。

THTM-His标记单抗可用于培养细胞的Western印迹分析、斑点印迹分析、ELISA、免疫荧光染色和流式细胞术。

二、His抗体A00186产品特性：

Host Species	Mouse
Conjugation	Unconjugated
Immunogen	A synthetic peptide HHHHHH coupled to KLH
Fusion Partner	Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells
Purification	Protein A affinity column

 

Subclass	IgG1, k
Clone ID	6G2A9
Concentration	0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity	THE™ His Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
Storage	The antibody is stable in lyophilized b if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Note	GenScript can offer this product according to your requirement, including product size, buffer components,etc.

三、产品应用：
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the b of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

四、公司介绍：

漠宝（厦门）生物科技有限公司是一家致力于生命科学和生物技术领域的企业，由厦门大学研究生创办。公司主要专注于生命科学科研试剂、耗材、仪器等科研用品的开发与销售。公司主要代理欧美著名生物技术公司的产品，能为生命科学多研究领域的科研人员提供全面的产品。我们相信经过几年的努力，漠宝生物一定能成为分子生物学、细胞生物学、免疫学等领域科研产品的供应商。

漠宝生物将以更好的客户服务、完备的产品供应、深厚的专业知识和优越的人脉资源，在同行业中保持良好的企业形象和竞争优势。我们将不断引进优质的产品，满足广大客户的需求，在世界各国大力发展生命科学的时代背景下，我们将以为“中国生命科学发展而尽上一份绵薄之力"为使命，为科学人员创造的科研成果提供强有力的支撑。

展望：公司将借助于厦门大学细胞应激生物学国家重点实验室的人才和技术优势，致力于研发、生产出品质优越的生命科学研究中用到的试剂、抗体、试剂盒等，力争在不久的将来将漠宝打造为生命科学领域的。

公司部分代理品牌--Genb（金斯瑞）、ExcellBio（依科赛）、SAB、Cyagen（赛业）、SBI、Bioteke（百泰克）、Crystalgen（科晶）、Solarbio（索莱宝）等。